The UV absorption spectrum of some solvents
overlaps with the product they dissolve, meaning
that fraction collection processes cannot distinguish
between solvent and product. Luckily, there is
technology that solves this problem.
Tags: Application Notes
Flash purification involves a simple liquid chromatography technique
» Method development uses TLC as a way of deciding the parameters for the
» Isocratic separations are easiest to develop, but gradient separations are
» Software in the Isolera helps with conversion of an isocratic separation to
» It is possible with the Spektra software to run step gradients
» Loading options are dependent on the column type
» SNAP offers the most flexibility
» Care must be taken to choose the best loading option to get good
Tags: Manuals & User Guides
Improvements in solid phase peptide synthesis strategies and
development of resin linkages susceptible to low acid cleavage
conditions has enabled synthesis of long peptides while keeping the
protecting groups intact. This strategy is now used for the
preparation of chemically synthesized proteins, wherein shorter
peptide fragments are ligated together. They are also found in the
synthesis of peptide macrocycles that utilize head-to-tail cyclization strategies. Although linear synthesis of protected peptides is generally straightforward, purification of these compounds using traditional reversed phase methods is quite challenging. Herein we describe the use of normal phase chromatography for purification of fully protected peptides.
Natural product chemistry deals with discovering the previously unknown in nature. Compounds found in nature are typically found in low quantity and thus extractions are needed to isolate certain compounds classes or at least compounds with similar solubility.
Tags: English, Posters
For most organic and natural product chemists flash
chromatography is a necessary part of their research. As such, many chemists need quick isolation of at least one desired component from a crude mixture in relatively high yield and purity. This need for speed, purity, and yield pits these desires against each other as you can typically optimize on only two of the three goals.
In this poster, we will describe some techniques that help chemists optimize flash purification and maximize speed, yield, and purity.
Tags: English, Posters
Although capable of very high resolution, RP-HPLC is often limited by low column loading capacity, therefore demanding a significant time investment for peptide purification. As an alternative strategy, reversed-phase flash chromatography can also be used to purify synthetic peptides. The larger particle size used in flash column chromatography enables much larger loading capacity, thereby significantly reducing the time required for peptide purification.
When it comes to polar organic compound purification,
many chemists turn to normal-phase flash
chromatography often utilizing dichloromethane and
methanol as the eluting solvents. While this can work, it
often can be challenging to optimize due to methanol’s
high polarity and protic chemistry.
Tags: English, Posters
As reversed-phase flash chromatography gains traction in
medicinal chemistry labs the need to monitor its cost and
safety are becoming more important. Commonly used
reversed-phase solvents typically include water with an
organic solvent such as methanol or acetonitrile – each
have advantages and disadvantages.
Tags: English, Posters
For those chemists performing organic synthesis, reaction mixture purification by flash column chromatography is an integral and necessary part of the synthesis process. However, flash chromatography consumes large volumes of solvent which either needs to be recycled or disposed.
Reversed-phase chromatography is typically used when you need to separate several milligrams of relatively polar compounds that either are not soluble in normal-phase solvents or are not compatible with bare silica
because they react, stick, or both. If you are currently using reversed-phase at preparative scale, such as flash chromatography, you know the mobile phase limitations – water with either methanol, acetonitrile, or
THF. As with normal-phase flash chromatography, when it comes time to purify you want your crude sample fully solubilized in the weakest possible solvent at the highest possible concentration.
DMSO and DMF are suitable injection solvents for reversed-phase flash purification. DMSO shows it can be loaded in larger volumes (up to 0.05 mL/g of C18 media or 3.5% of a column volume) without affecting chromatographic separations or carrying compounds with it.
Using the Biotage automated workflow, models have shown that the time taken to produce a target molecule can be reduced by up to eighty percent, allowing project delivery timescales to be reduced
Tags: Product Notes
Traditional approaches to compound purification involving chromatography utilize large volumes of relatively toxic and expensive solvents, and significantly contribute to the environmental footprint of organizations involved in molecular research. Current directives for greener chemistry have put pressure on organizations to reduce the
environmental impact of their work.
Tags: Environmental, Product Notes
The adapter kit contains a pre-assembled stand, spacer and
connecting tubing; everything required to allow the use of
SNAP 340g cartridges on flash purification systems that utilize
Luer tubing connections such as, Isco Companion and Analogix
Tags: English, Product Notes
Biotage® SNAP Ultra flash cartridges allow you to load your samples in seven different ways. No matter what you are purifying - there is always a solution to get the best possible separati...06 August 2014
Fatty acids and their esters are found in many biological substrates. Many of these fats are unhealthy (e.g. saturated fats) while others have been found to be beneficial (e.g. unsaturated, polyuns...29 July 2014
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
Isolera™ Prime is a value based system which recommends appropriate cartridges and calculates the best solvent conditions and gradients for your purifications. It has solvent pumps, automated fraction collection as standard and delivers much more than just the basics. Solvent use is minimized and advanced compound detection makes sure that your purified compound is delivered at the end of the run. We belive in providing value, so Isolera Prime systems embrace ACI™, a revolutionary advancement, converting regular flash from simple purification to the fastest, greenest, and most economical way to reliably isolate pure compounds for organic synthesis. ACI offerred as standard on Isolera Prime.
Isolera™ Dalton is the most advanced and innovative flash purification system available, designed by people who understand flash chromatography for the chemists who need it.
"Pure convenience! Just pure convenience! It lets us perform purification and analysis at the same time. It gives us tremendous power to isolate unknown compounds.”
- Hiroki Nishii, Chugai Pharmaceutical’s Kamakura Research Laboratories
|Part No.||Description||Product type||Pack Size||Price|
|120-1110||Flash Luer Lock to Luer Adapters||Accessories||25||Log in for price|
|411069||FlashMaster II Holder||Accessories||1||Log in for price|
|412358||XL Fitting, Female Luer, Assembly - Isolera LS||Accessories||1||Log in for price|
|412537||Male Luer outlet fitting for SNAP 750g and 1500g cartridges||Accessories||1||Log in for price|
|413027||3-way large bore stainless steel injection valve for Isolera systems||Accessories||1||Log in for price|
|413092||Biotage ZIP 5g/10g cartridge holder||Accessories||1||Log in for price|
|413302||Biotage ZIP 30g cartridge holder, 1/cs||Accessories||1||Log in for price|
|413303||Biotage ZIP 45g cartridge holder, 1/cs||Accessories||1||Log in for price|
|413304||Biotage ZIP 80g cartridge holder, 1/cs||Accessories||1||Log in for price|
|413305||Biotage ZIP 120g cartridge holder, 1/cs||Accessories||1||Log in for price|