Samplet® Cartridges

The UV absorption spectrum of some solvents overlaps with the product they dissolve, meaning that fraction collection processes cannot distinguish between solvent and product. Luckily, there is technology that solves this problem.
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Normal-phase flash chromatography1 has been widely adopted as the method of choice for separation of product mixtures and reaction by-products. One of the most significant developments in this area concerns the practical separation of polar molecules. Reversed-phase purification is a modification of normalphase chromatography that provides an efficient mechanism for the separation of polar compounds.
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Biotage has developed tools for every step of the organic process, with the entire workflow in mind. This dedicated suite of products vastly expands the range of options in order to truly accelerate discoveries of new molecules for future innovations.
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Flash purification is a separation technique developed in 1978 by Professor W.C. Still that uses a stationary phase (a column or cartridge filled with an insoluble solid support) and a mobile phase (elution solvent mixture) to separate and purify a mixture of organic compounds.
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Normal-phase flash chromatography1 has been widely adopted as the method of choice for separation of product mixtures and reaction by-products. One of the most significant developments in this area concerns the practical separation of polar molecules. Reversed-phase purification is a modification of normalphase chromatography that provides an efficient mechanism for the separation of polar compounds.
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The definitive guide to flash chromatography. This document presents in detail the broad selection of ways that materials for purification can be loaded onto a flash cartridge.
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Flash purification involves a simple liquid chromatography technique » Method development uses TLC as a way of deciding the parameters for the separation » Isocratic separations are easiest to develop, but gradient separations are more powerful » Software in the Isolera helps with conversion of an isocratic separation to a gradient » It is possible with the Spektra software to run step gradients » Loading options are dependent on the column type » SNAP offers the most flexibility » Care must be taken to choose the best loading option to get good purifications
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Normal-phase flash chromatography1 has been widely adopted as the method of choice for separation of product mixtures and reaction by-products. One of the most significant developments in this area concerns the practical separation of polar molecules. Reversed-phase purification is a modification of normalphase chromatography that provides an efficient mechanism for the separation of polar compounds.
Tags: Application Notes, Brochures, English, Manuals & User Guides

For those chemists performing organic synthesis, reaction mixture purification by flash column chromatography is an integral and necessary part of the synthesis process. However, flash chromatography consumes large volumes of solvent which either needs to be recycled or disposed. ACS 2016.
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Reversed-phase chromatography is typically used when you need to separate several milligrams of relatively polar compounds that either are not soluble in normal-phase solvents or are not compatible with bare silica because they react, stick, or both. If you are currently using reversed-phase at preparative scale, such as flash chromatography, you know the mobile phase limitations – water with either methanol, acetonitrile, or THF. As with normal-phase flash chromatography, when it comes time to purify you want your crude sample fully solubilized in the weakest possible solvent at the highest possible concentration. ACS 2016
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DMSO and DMF are suitable injection solvents for reversed-phase flash purification. DMSO shows it can be loaded in larger volumes (up to 0.05 mL/g of C18 media or 3.5% of a column volume) without affecting chromatographic separations or carrying compounds with it.
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To save money on consumables, many chemists choose to reuse silica flash cartridges. This is true but risks purification results because chromatographic separation performance will change from run to run which reduces purification quality, especially in normal phase systems. Regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and therefore fraction purity.
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With running costs and time a concern in most labs, ACI™ now promises to take users to the end point even faster and even more efficiently.
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Reversed-phase flash chromatography is a very popular purification technique using a non-polar stationary phase. Main application areas include separation of polar, ionizable and highly lipophilic compounds which cannot easily be separated by normal-phase techniques.
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Biotage SNAP cartridges can be used on any flash system. The easy-to-use Luer-lok® connections do not need a compression module to operate at 100 psi. Made from USP Class VI materials, Biotage SNAP cartridges offer 7 types of loading techniques including 3 internal dry loading options. Packed to provide the highest plate count of any 50μm particle cartridge on the market, SNAP provides increased loading capacity and better compound separations.
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