Committed to preserving our shared environment – further details available on request.
Manufacture of consumables is accredited by the world renowned British Standards Institute.
Flexible supply chains, back up manufacturing and warehousing ensures supply risk reduction
Products which are scaleable from lab or development to process scale.
Can be used in processes in compliance with GLP standards.
For more information download the Industrial Product Portfolio Catalog.
Selecting the Correct Stationary Phase
Normal phase flash chromatography has been widely adopted as the method of choice for separation of product mixtures and reaction by-products. Whether standard silica or specially acid washed for low metals content, normal phase flash purification has proven itself to be incredibly powerful. One of the most significant developments in purification is the separation of polar molecules however, is by reversed phase purification. Reversed phase methods provide a great leap forward, but this has been a relatively under-used technique due to lack of information and supporting data. Polar, water soluble molecules are the focus of many pharmaceutical drug development programs and natural product research. These molecules make up the majority of the compounds involved in the fundamental chemistry of living organisms.
Application Selection Guide
Choice of Silica and Stationary Phase
Isolation of large quantities of such polar compounds has traditionally been carried out using expensive preparative HPLC (High Performance Liquid Chromatography) systems. These systems are typically dedicated to final product purification, and are not economically viable options for many chemists in day to day or smaller/flexible projects. The range of different chemistries served by Biotage stationary phases is shown below. Additionally, Biotage offers a custom packing service for other stationary phases, please enquire for further details.
Cartridge Media Specifications
How to Scale Up and Select an Industrial Purification System and Cartridge
Moving from lab scale to large scale comes with a number of additional considerations. Biotage® Flash 75, 150 and 400 systems are extremely tough, economically viable industrial purification systems that have been developed for this purpose. Scaling up flash purification methods is easy and straightforward. Any method developed using a Biotage lab scale cartridge can be transferred to a larger cartridge using the scale-up factors in the table below. Once the development cartridge is determined, the lab scale method can be replayed, providing peak elution profiles for the scale up system consistent with the development process and suitable for final optimization. Furthermore, all Biotage lab scale systems come with software that can automatically create linear gradients from TLC Rf spots; and then automatically convert those linear gradients into step gradients for application on a larger scale. Each system may be configured with either ‘M’ or ‘L’ cartridge capability to further increase its flexibility and range.
A 25 g Biotage® SNAP cartridge was used to develop a 2.3 gram purification. The requirement is now to purify 125 g. The scale-up factor is then 54.3. We therefore move right in the chart on the 25 g row to the interval between 32 and 60. The appropriate large scale cartridge is in the 800–2500 g range, which corresponds to the Biotage® Flash 150M cartridge.
A 100 g Biotage® SNAP cartridge was used to develop a 6.5 gram purification. The requirement is now to purify 900 g. The scale-up factor is then 138. We therefore move right in the chart on the 100 g row to the interval between 50 and 200. The appropriate large scale cartridge is in the 5–20 kg range, which corresponds to the Biotage® Flash 400M cartridge.
Cartridge Scale-up Conversion Table
Reduce Purification Time and Cartridge Size with Higher Capacity Cartridges
Traditional process purification can benefit from advances in lab scale purification and materials science. Classic selection and predictor tables are based on a standard performance silica media for determining cartridge loading and sample size. Biotage® HP-Sphere™ and KP-Sphere™ represent an innovative breakthrough in purification technology, leading to very high capacity and high resolution purification columns. With spherical beads, smaller particle size and a much higher surface area, these silica are more efficiently packed into columns, increasing the effective plate count whilst conferring approximately double the sample load capability. These revolutionary improvements result in a column that doubles the purification performance per run or allows a smaller column to be chosen for the same sample quantity, cutting the solvent use and run times in half.