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ISOLUTE® SCX-2
SPE Columns and Plates
ISOLUTE® SCX-2 is a strong cation exchange sorbent with minimal non-polar character, used to extract basic analytes from aqueous samples.
| Chemical description | Propylsulfonic acid (non-endcapped) functionalized silica. manufactured using a trifunctional silane. |
| Sorbent type | Strong cation exchange |
| Average particle size | 50 µm |
| Pore diameter | 60 Å |
| Exchange capacity | 0.6 meq/g |
» APPLICATION NOTES
Increasing the Selectivity of Silica Based Cation Exchange Sorbents for the Purification of Reaction Mixtures Using Derivatization Purification Techniques
This technical note describes the use of derivatization techniques to separate aliphatic
secondary amines from aliphatic tertiary amines in a mixture using the strong cation
exchange sorbent, ISOLUTE® SCX-2.
Tags: Application Notes,
Product Notes
» CATALOGS
Analytical Sample Preparation 2018 Catalog
In this catalog product information is displayed with easy-to-follow application details and supporting documentation.
Ordering information is found following each product listing.
Tags: Catalogs
» MANUALS & USER GUIDES
QuickStart Guide to SPE
QuickStart Guide to SPE
Tags: English,
Manuals & User Guides
Step By Step Guide To SPE Method Development
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
» MATERIAL SAFETY DATA SHEETS
Safety Data Sheet SDS 1.0 ISOLUTE SCX-2/SAX
Laboratory chemical
Not for therapeutic or diagnostic use
Tags: English,
Material Safety Data Sheets
Safety Data Sheet ISOLUTE SCX– 2
ISOLUTE S. C. X. – 2
9532
Laboratory chemical
Tags: English,
Material Safety Data Sheets
» POSTERS
Ion Exchange Impurity Scavenging Improves Flash Purification Efficiency
In medicinal chemistry, organic synthesis will generate by-products with similar chemistry to the product. Separating these impurities from the product often becomes a challenge that often only prep HPLC can achieve.
In this poster, we discuss how using sample dry loading with a cation exchange scavenger media with flash chromatography
improves product purity by removing many of the impurities.
Tags: Posters
» PRODUCT NOTES
Method Development Guidelines: Solid Phase Extraction using ISOLUTE® SCX-2 SPE Sorbents for the Extraction of Aqueous Samples
The ISOLUTE® family of cation exchange sorbents are used to extract organic cations (basic compounds capable of exhibiting a positive charge) from both aqueous and non-aqueous matrixes. Although extraction is by the same mechanism, each sorbent has properties that influence the way they are used.
Tags: English,
Product Notes
Increasing the Selectivity of Silica Based Cation Exchange Sorbents for the Purification of Reaction Mixtures Using Derivatization Purification Techniques
This technical note describes the use of derivatization techniques to separate aliphatic
secondary amines from aliphatic tertiary amines in a mixture using the strong cation
exchange sorbent, ISOLUTE® SCX-2.
Tags: Application Notes,
Product Notes
Work-up of Basic Products from Organic Synthesis Mixtures using ISOLUTE® SCX-2
In medicinal chemistry reactions are often carried out in solvents such as DMSO. Once the reaction is complete, it is usually necessary to separate the products of the reaction from reagents and by-products. This can be performed using strong cation exchange sorbents (e.g. ISOLUTE SCX-2 or ISOLUTE Flash SCX-2) to selectively isolate the basic compounds from the reaction mixture. (see Figure 1 for structure of ISOLUTE SCX-2 and ISOLUTE Flash SCX-2 sorbents)
Tags: Product Notes
ISOLUTE® Si-Propylsulfonic Acid (SCX-2) Scavenger/Reagent
ISOLUTE® Si-Propylsulfonic acid (SCX-2) belongs to the class of strong acids. Similar
to ISOLUTE Si-TsOH (SCX-3)1, it can be used to (a) scavenge amines and other bases such
as anilines and borohydrides (b) as an acid catalyst in reactions or (c) as a replacement
for aqueous or organic acids in quenching reactions. The advantage of using SCX-2 as a
bound acid reagent is in that it eliminates work-up. The crude reaction mixture can be evaporated to dryness and the resulting free flowing solid can be purified directly by flash column chromatography.
Tags: Product Notes
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News
11 January 2018The 2018 Analytical Catalog is Here
The Biotage 2018 catalog of analytical sample preparation products for chemistry professionals is now available. This edition includes a comprehensive range of sample preparation consumables for bi...
17 July 2017Biotage Goes Direct in Italy
On July 1st, Biotage expanded its direct sales organization and opened the door to analytical customers in Italy. Account Manager Gianluca Pessoni is added to the Italian team, filling the ana...
28 June 2017Extraction of Tobacco-Specific Nitrosamines (TSNAs) from Urine Using ISOLUTE® SLE+ Prior to UPLC/MS/MS Analysis
This application note from Biotage Labs describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sampl...
15 June 2017Extraction of Amphetamines and Analogues from Hair Using ISOLUTE® SLE+ Prior to GC/MS Analysis
This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. This application note describe...
20 March 2017Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil
This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...
20 February 2017Extraction of 49 Illicit Drugs of Abuse from Whole Blood Using ISOLUTE® SLE+
This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized the for ...
05 January 20162016 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
11 August 2015Why Dilute and Shoot Ruins Your Results
Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...
27 March 2015New Video – Thirty Years of Packing Flash Columns
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...
08 January 20152015 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
29 July 2014Free Shipping on EU Web Orders
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
Q&A
SPE column capacity
01 July 2013Question:
What is the maximum capacity of an SPE column?
Answer:
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.
Browse more questions and answers >CONSUMABLES
| Part No. | Description | Product type | Pack Size | Price | ||
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532-0002-A | ISOLUTE® SCX-2 25 mg/1 mL | Consumables | 100 | Log in for price | |
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532-0005-A | ISOLUTE® SCX-2 50 mg/1 mL | Consumables | 100 | Log in for price | |
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532-0010-A | ISOLUTE® SCX-2 100 mg/1 mL | Consumables | 100 | Log in for price | |
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532-0010-B | ISOLUTE® SCX-2 100 mg/3 mL | Consumables | 50 | Log in for price | |
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532-0020-B | ISOLUTE® SCX-2 200 mg/3 mL | Consumables | 50 | Log in for price | |
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532-0050-B | ISOLUTE® SCX-2 500 mg/3 mL | Consumables | 50 | Log in for price | |
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532-0050-C | ISOLUTE® SCX-2 500 mg/6 mL | Consumables | 30 | Log in for price | |
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532-0050-H | ISOLUTE® SCX-2 500 mg/10 mL (3 mL XL) | Consumables | 50 | Log in for price | |
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532-0100-B | ISOLUTE® SCX-2 1 g/3 mL | Consumables | 50 | Log in for price | |
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532-0100-C | ISOLUTE® SCX-2 1 g/6 mL | Consumables | 30 | Log in for price | |
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532-0200-D | ISOLUTE® SCX-2 2 g/15 mL | Consumables | 20 | Log in for price | |
ISOLUTE® SCX-2 is a propylsulfonic acid bonded sorbent used for the extraction of basic compounds from aqueous (or partially aqueous) samples using a strong cation exchange retention mechanism. It keeps a permanent negative charge across the pH range. Analytes are eluted from ISOLUTE SCX-2 by eliminating the charge on the analyte (high pH), so this sorbent is not suitable for extraction of strong bases. The sorbent is supplied in the protonated form.
ISOLUTE SCX-2 is the sorbent of choice when strong cation exchange is the primary retention mechanism, or if aqueous conditions are required for analyte elution. For methods listing PRS as the SPE sorbent, ISOLUTE SCX-2 is a direct replacement.
ISOLUTE SCX-2 is available in a wide range of SPE columns and 96-well plate formats.
