
"Simultaneous detection of both positive and negative ions is fantastic. Rarely do samples contain just positively or negatively charged species."
Detection range (m/z) | 10–2000 |
Ion source | APCI (ESI optional) |
Resolution | < 0.7 FWHM m/z |
Detection accuracy | ±0.1 m/z |
Acquisition modes | Switching (positive and negative) |
Flow rate range | Flash separation at 10–100 mL/min |
Sampling adjustment | Automatic split flow interface |
Mass fractionation limit | 4 |
Collect on TIC | Yes |
Custom mass intervals | Yes |
Automatic stop on target mass | Yes |
Post run analysis | Built into software and offline |
Solvents | One make-up solvent (pure) |
Nitrogen gas pressure | 4.1–6.9 bar (60–100 psi), minimum 98% purity |
Gas consumption | 8 L/min |
Control interface | Isolera™ Spektra |
Accessories | ASAP direct probe analysis |
User serviceable parts | Heated inlet capillary with o-ring and Corona needle |
Vacuum pumps | Internal turbo pump and external rotary vane roughing pump |
Dimensions (w x d x h) | 28 x 56 x 66 cm |
Weight | 54 kg |
Certifications | CE, TÜV, FCC |
Reversed Phase chromatography using Isolera Dalton 2000
Tags: Application Notes
The UV absorption spectrum of some solvents
overlaps with the product they dissolve, meaning
that fraction collection processes cannot distinguish
between solvent and product. Luckily, there is
technology that solves this problem.
Tags: Application Notes
This application note outlines how to use the Isolera system to perform a size-exclusion separation as part of a sample clean up method defined by the EPA, a key sample preparation step in environmental laboratories.
Tags: Application Notes
Summary of Biotage offerings for Mass Directed Flash Chromatography
Tags: Brochures
Biotage has developed a range
of tools to improve the peptide synthesis workflow,
from synthesis of crude peptides through to the final
purified product. These solutions make your workflow
more efficient and improve the quality of peptides
you synthesize. Our workflow solutions are used in
pharma, biotech, CRO and academic laboratories
throughout the world.
Tags: Brochures
Biotage has developed tools for every
step of the organic process, with the entire workflow in mind.
This dedicated suite of products vastly expands the
range of options in order to truly accelerate discoveries
of new molecules for future innovations.
Tags: Brochures
This brochure presents the extensive range of instrumentation for flash purification from Biotage. To complement these products Biotage also offers a complete range of flash consumables, including columns in a variety of sizes packed with irregular and spherical silica, making Biotage your one-stop partner for your flash purification needs.
Keywords: Isolera™ Dalton, Isolera™ Spektra, 10 Isolera™ Spektra One and Four, Isolera™ Spektra LS (Large Scale), Isolera™ Prime, Isolera™ ELSD-1080, Biotage® Flash 75/150, Biotage® Flash 400
Tags: Brochures
How to use the Atmospheric Solid Analysis Probe on Dalton 2000
Tags: Manuals & User Guides
How to Install Isolera SW 2.3
Tags: Manuals & User Guides
Flash purification involves a simple liquid chromatography technique
» Method development uses TLC as a way of deciding the parameters for the
separation
» Isocratic separations are easiest to develop, but gradient separations are
more powerful
» Software in the Isolera helps with conversion of an isocratic separation to
a gradient
» It is possible with the Spektra software to run step gradients
» Loading options are dependent on the column type
» SNAP offers the most flexibility
» Care must be taken to choose the best loading option to get good
purifications
Tags: Manuals & User Guides
Preparation, How Much are You Purifying?
Tags: Manuals & User Guides
The Isolera leak detection system is designed with safety in
mind. Unlike vapor sensing devices with unreliable solvent
vapor sensitivity, the Isolera leak detector’s sensitive,
solvent-resistant refractive index (RI) monitor rapidly
signals the Isolera flash system to stop pumping if any
liquid, volatile or non-volatile, is detected thus protecting
the chemist, system, and lab from potential hazards.
Tags: Manuals & User Guides,
Product Notes
Improvements in solid phase peptide synthesis strategies and
development of resin linkages susceptible to low acid cleavage
conditions has enabled synthesis of long peptides while keeping the
protecting groups intact. This strategy is now used for the
preparation of chemically synthesized proteins, wherein shorter
peptide fragments are ligated together. They are also found in the
synthesis of peptide macrocycles that utilize head-to-tail cyclization strategies. Although linear synthesis of protected peptides is generally straightforward, purification of these compounds using traditional reversed phase methods is quite challenging. Herein we describe the use of normal phase chromatography for purification of fully protected peptides.
Tags: Posters
Natural product chemistry deals with discovering the previously unknown in nature. Compounds found in nature are typically found in low quantity and thus extractions are needed to isolate certain compounds classes or at least compounds with similar solubility.
Tags: English,
Posters
For most organic and natural product chemists flash
chromatography is a necessary part of their research. As such, many chemists need quick isolation of at least one desired component from a crude mixture in relatively high yield and purity. This need for speed, purity, and yield pits these desires against each other as you can typically optimize on only two of the three goals.
In this poster, we will describe some techniques that help chemists optimize flash purification and maximize speed, yield, and purity.
Tags: English,
Posters
Although capable of very high resolution, RP-HPLC is often limited by low column loading capacity, therefore demanding a significant time investment for peptide purification. As an alternative strategy, reversed-phase flash chromatography can also be used to purify synthetic peptides. The larger particle size used in flash column chromatography enables much larger loading capacity, thereby significantly reducing the time required for peptide purification.
Tags: Posters
When it comes to polar organic compound purification,
many chemists turn to normal-phase flash
chromatography often utilizing dichloromethane and
methanol as the eluting solvents. While this can work, it
often can be challenging to optimize due to methanol’s
high polarity and protic chemistry.
Tags: English,
Posters
As reversed-phase flash chromatography gains traction in
medicinal chemistry labs the need to monitor its cost and
safety are becoming more important. Commonly used
reversed-phase solvents typically include water with an
organic solvent such as methanol or acetonitrile – each
have advantages and disadvantages.
Tags: English,
Posters
For those chemists performing organic synthesis, reaction mixture purification by flash column chromatography is an integral and necessary part of the synthesis process. However, flash chromatography consumes large volumes of solvent which either needs to be recycled or disposed.
ACS 2016.
Tags: Posters
Reversed-phase chromatography is typically used when you need to separate several milligrams of relatively polar compounds that either are not soluble in normal-phase solvents or are not compatible with bare silica
because they react, stick, or both. If you are currently using reversed-phase at preparative scale, such as flash chromatography, you know the mobile phase limitations – water with either methanol, acetonitrile, or
THF. As with normal-phase flash chromatography, when it comes time to purify you want your crude sample fully solubilized in the weakest possible solvent at the highest possible concentration.
ACS 2016
Tags: Posters
DMSO and DMF are suitable injection solvents for reversed-phase flash purification. DMSO shows it can be loaded in larger volumes (up to 0.05 mL/g of C18 media or 3.5% of a column volume) without affecting chromatographic separations or carrying compounds with it.
Tags: Posters
New, higher default flow rates enabled by Isolera ACI systems decreases purification times compared to other systems’ and cartridges’ default flow rate settings without sacrificing separation efficiency. Incorporation of gradient optimization by converting a linear gradient to a step gradient further reduces purification time and solvent use while maintaining separation integrity for a target molecule.
Tags: Posters
In this poster we demonstrate real-time detection and identification of peptides during reversed-phase flash chromatography with the new Biotage® Isolera™ Dalton mass-directed flash purification system.
Presented at ACS Spring 2014.
Tags: Posters
In order to obtain a fast synthesis of an N-unprotected tripeptide, microwave (mw) assisted deprotection, coupling and partial deprotection were applied starting with a purified protected dipeptide [Fig. 1]. The three consecutive mw assisted steps were performed without any intermediate purifications and were accomplished within some 30 minutes. The resulting crude mixture containing the Boc deprotected tripeptide ester was shown to be quite complex [Fig. 2]. The crude product was subjected to automated reversed phase flash chromatography, followed by automated fraction pooling and “thin film evaporation”. The total time for the entire procedure was 1 h 20 min. The purity of the product was > 99%.
Tags: Posters
What is included in the Installation and Familiarization pachage for Isolera™ Dalton 2000?
Tags: Product Notes
Using the Biotage automated workflow, models have shown that the time taken to produce a target molecule can be reduced by up to eighty percent, allowing project delivery timescales to be reduced
accordingly.
Tags: Product Notes
Traditional approaches to compound purification involving chromatography utilize large volumes of relatively toxic and expensive solvents, and significantly contribute to the environmental footprint of organizations involved in molecular research. Current directives for greener chemistry have put pressure on organizations to reduce the
environmental impact of their work.
Tags: Product Notes
With running costs and time a concern in most labs, ACI™ now promises to take users to the end point even faster and even more efficiently.
Tags: Product Notes
The Isolera leak detection system is designed with safety in
mind. Unlike vapor sensing devices with unreliable solvent
vapor sensitivity, the Isolera leak detector’s sensitive,
solvent-resistant refractive index (RI) monitor rapidly
signals the Isolera flash system to stop pumping if any
liquid, volatile or non-volatile, is detected thus protecting
the chemist, system, and lab from potential hazards.
Tags: Manuals & User Guides,
Product Notes
Use this simple tool to look up possible adducts and fragments which may occur with Isolera™ Dalton or Biotage® Dalton 2000 mass detectors.
Enter the target compound weight and use the mass lookup cell to highlight resulting m/z values.
Tags: Software
In-line mass detection has become a powerful addition to the flash chemist's toolbox. While this technical advance in flash chromatography has helped chemists detect and isolate pure compounds...
13 July 2017Biotage is pleased to announce the release of the new Peptide Synthesis Workflow – Synthesis, Purification, and Evaporation Solutions Brochure The typical peptide synthesis workflow broadly ...
24 May 2017Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, is pleased to announce the launch of Isolera™ Dalton 2000, a new ma...
12 September 2014The capabilities of Biotage flash chromatography systems for providing pure compounds from natural products is confirmed in an extensive Korean study of active compounds in the medicinal herb&...
09 September 2014ACI™ - Accelerated Chromatographic Isolation radically improves the efficiency of the laboratory, embracing the latest developments in purification technology to guide chemists towards improv...
07 August 2014Flash Purification Has Come of Age Flash purification is a chromatographic technique developed with one goal in mind – to deliver the maximum amount of purified product in the minimum ...
27 June 2014Uppsala, Sweden, 27th June, 2014 – Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, announces the launch of ACI&trad...
14 March 2014By Dr. Greg Saunders, EU Marketing Manager for Organic and Peptide Chemistry at Biotage Liquid chromatography (LC) is used for two different purposes – analytical work and purification &ndas...
11 February 2013Uppsala, Sweden, 1st February, 2013 Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, has announced the launch of an updat...
04 September 2012Uppsala, Sweden, 4th September, 2012 Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, announces that Biotage is in late s...
15 June 2012Dr. Sunil Rana, product manager at Biotage, explains the advanced features for flash chromatography in the new Isolera™ Spektra release. Watch the free webinar recording here >
26 April 201223 April 2012 Product manager Bob Bickler introduces the new time-saving Isolera™ Spektra features for Isolera One, Four and LS Flash chromatography instruments.
18 October 2011Preparative FLASH chromatography expedites the production of clinical trial compounds. Tobi Williams is an application chemist specializing in pilot and production purification at Biotag...
16 August 2010Charlotte, North Carolina, USA, August 16, 2010 – Biotage (STO: BIOT), a leading developer of tools and technology for medicinal and analytical chemistry, announces the introduction of the Is...
21 April 2010Uppsala, Sweden, April 20, 2010 – Biotage (STO: BIOT), a global leader in automated flash purification, tools and technology for medicinal and analytical chemistry, announced it has recently ...
23 February 2010Uppsala, Sweden, February 23, 2010 – Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, announced the launch of enhanced gradient optimization (GO) te...
27 October 2008October 28, 2008 Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, announces the release of its new 2008–09 Synthesis and Purification Catalog. The ...
Part No. | Description | Product type | Pack Size | Price | ||
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DALT-2000-APCI-100V | Biotage Dalton 2000 Mass Detector APCI 100 VAC incl roughing pump | Instruments | 1 | Log in for price | |
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DALT-2000-APCI-115V | Biotage Dalton 2000 Mass Detector APCI 115 VAC incl roughing pump | Instruments | 1 | Log in for price | |
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DALT-2000-APCI-230V | Biotage Dalton 2000 Mass Detector APCI 230 VAC incl roughing pump | Instruments | 1 | Log in for price |
Part No. | Description | Product type | Pack Size | Price | ||
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DALT-2000-NLINK | Biotage Dalton 2000 Nanolink | Accessories | 1 | Log in for price | |
IS-ESI-S01 | Electrospray Ionization (ESI) ion source | Accessories | 0 | Log in for price | ||
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UP-ASAP-SNL | Dalton 2000 ASAP kit, probe, capillary stand, APCI source port | Accessories | 1 | Log in for price |
The Isolera™ Dalton 2000 system consists of Biotage® Dalton 2000 Mass Detector, Isolera™ Dalton Nanolink and Isolera™ Spektra and embraces ACI™ for efficient and automated purification. Isolera Dalton 2000 directs flash fractionation by molecular mass, and in real time.
Biotage® Dalton 2000 is a compact single quadrupole mass detector with simultaneous positive and negative polarity switching. Biotage® Dalton 2000 uses an atmospheric pressure chemical ionization source to ionize substances, which can then be identified by their mass-to-charge ratios (m/z). Biotage® Dalton 2000 allows you to analyze samples and automatically use the m/z ratio(s) of the identified substance(s) for flash purification.
A novel function, 'Collect on TIC', monitors the entire specified range of m/z values. This is beneficial for samples that easily form fragments and/or adducts, where peaks are therefore found on several m/z values, or if the sample contains many different components.
Isolera™ Dalton 2000 automates and takes the hard work out of the compound identification and purification, perfect for chemists whose priorities lie more towards the chemistry than small details of running the analysis. Minimal user intervention required makes it available to chemists unfamiliar with mass detection or those whose priorities lie in compound synthesis rather than analysis.
Seamless interaction between a flash system and a mass detector requires advanced technology and fine tuning. This is all taken care of by the Isolera™ Dalton Nanolink interface, an intelligent sampling device that at any given moment provides Biotage® Dalton 2000 Mass Detector with the exact amount of sample required, and dynamically adjusts to the solvent effluent flow rate employed in the Flash system.
Isolera™ Spektra software with its graphic user interface provides a straightforward controlling environment, giving easy access to the process.