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Isolera™ Dalton 2000
Bringing Mass Detection to Flash Chromatography
"Simultaneous detection of both positive and negative ions is fantastic. Rarely do samples contain just positively or negatively charged species."
| Detection range (m/z) | 10–2000 |
| Ion source | APCI (ESI optional) |
| Resolution | < 0.7 FWHM m/z |
| Detection accuracy | ±0.1 m/z |
| Acquisition modes | Switching (positive and negative) |
| Flow rate range | Flash separation at 10–100 mL/min |
| Sampling adjustment | Automatic split flow interface |
| Mass fractionation limit | 4 |
| Collect on TIC | Yes |
| Custom mass intervals | Yes |
| Automatic stop on target mass | Yes |
| Post run analysis | Built into software and offline |
| Solvents | One make-up solvent (pure) |
| Nitrogen gas pressure | 4.1–6.9 bar (60–100 psi), minimum 98% purity |
| Gas consumption | 8 L/min |
| Control interface | Isolera™ Spektra |
| Accessories | ASAP direct probe analysis |
| User serviceable parts | Heated inlet capillary with o-ring and Corona needle |
| Vacuum pumps | Internal turbo pump and external rotary vane roughing pump |
| Dimensions (w x d x h) | 28 x 56 x 66 cm |
| Weight | 54 kg |
| Certifications | CE, TÜV, FCC |
» APPLICATION NOTES
Dalton 2000 Run-Report 2017-May-11 10.39
Reversed Phase chromatography using Isolera Dalton 2000
Tags: Application Notes
Flash Chromatography: Using UV Detection with UV Absorbing Mobile Phases
The UV absorption spectrum of some solvents
overlaps with the product they dissolve, meaning
that fraction collection processes cannot distinguish
between solvent and product. Luckily, there is
technology that solves this problem.
Tags: Application Notes
Environmental Sample Clean-up on Isolera™ by Size Exclusion Chromatography
This application note outlines how to use the Isolera system to perform a size-exclusion separation as part of a sample clean up method defined by the EPA, a key sample preparation step in environmental laboratories.
Tags: Application Notes
» BROCHURES
Mass Directed Flash Chromatography by Biotage - Dalton Mass Detectors
Summary of Biotage offerings for Mass Directed Flash Chromatography
Tags: Brochures
Peptide Synthesis Workflow - Synthesis, Purification, and Evaporation Solutions
Biotage has developed a range
of tools to improve the peptide synthesis workflow,
from synthesis of crude peptides through to the final
purified product. These solutions make your workflow
more efficient and improve the quality of peptides
you synthesize. Our workflow solutions are used in
pharma, biotech, CRO and academic laboratories
throughout the world.
Tags: Brochures
Organic Chemistry - Products from Start to Finish
Biotage has developed tools for every
step of the organic process, with the entire workflow in mind.
This dedicated suite of products vastly expands the
range of options in order to truly accelerate discoveries
of new molecules for future innovations.
Tags: Brochures
Biotage Flash Purification Systems - Pure Compounds in a Flash
This brochure presents the extensive range of instrumentation for flash purification from Biotage. To complement these products Biotage also offers a complete range of flash consumables, including columns in a variety of sizes packed with irregular and spherical silica, making Biotage your one-stop partner for your flash purification needs.
Keywords: Isolera™ Dalton, Isolera™ Spektra, 10 Isolera™ Spektra One and Four, Isolera™ Spektra LS (Large Scale), Isolera™ Prime, Isolera™ ELSD-1080, Biotage® Flash 75/150, Biotage® Flash 400
Tags: Brochures
» MANUALS & USER GUIDES
ASAP installation and user instruction
How to use the Atmospheric Solid Analysis Probe on Dalton 2000
Tags: Manuals & User Guides
Isolera Software Upgrade Instructions
How to Install Isolera SW 2.3
Tags: Manuals & User Guides
Method Development in Flash Purification
Flash purification involves a simple liquid chromatography technique
» Method development uses TLC as a way of deciding the parameters for the
separation
» Isocratic separations are easiest to develop, but gradient separations are
more powerful
» Software in the Isolera helps with conversion of an isocratic separation to
a gradient
» It is possible with the Spektra software to run step gradients
» Loading options are dependent on the column type
» SNAP offers the most flexibility
» Care must be taken to choose the best loading option to get good
purifications
Tags: Manuals & User Guides
Quick Guide to Isolera™
Preparation, How Much are You Purifying?
Tags: Manuals & User Guides
Isolera™ Leak Detection System
The Isolera leak detection system is designed with safety in
mind. Unlike vapor sensing devices with unreliable solvent
vapor sensitivity, the Isolera leak detector’s sensitive,
solvent-resistant refractive index (RI) monitor rapidly
signals the Isolera flash system to stop pumping if any
liquid, volatile or non-volatile, is detected thus protecting
the chemist, system, and lab from potential hazards.
Tags: Manuals & User Guides,
Product Notes
» POSTERS
Options Available for Green Flash Chromatography
For those chemists performing organic synthesis, reaction mixture purification by flash column chromatography is an integral and necessary part of the synthesis process. However, flash chromatography consumes large volumes of solvent which either needs to be recycled or disposed.
ACS 2016.
Tags: Posters
Impact of Injection Solvent Choice on Peak Shape and Resolution in Reversed-phase Flash Chromatography
Reversed-phase chromatography is typically used when you need to separate several milligrams of relatively polar compounds that either are not soluble in normal-phase solvents or are not compatible with bare silica
because they react, stick, or both. If you are currently using reversed-phase at preparative scale, such as flash chromatography, you know the mobile phase limitations – water with either methanol, acetonitrile, or
THF. As with normal-phase flash chromatography, when it comes time to purify you want your crude sample fully solubilized in the weakest possible solvent at the highest possible concentration.
ACS 2016
Tags: Posters
DMSO and DMF as Injection Solvents
DMSO and DMF are suitable injection solvents for reversed-phase flash purification. DMSO shows it can be loaded in larger volumes (up to 0.05 mL/g of C18 media or 3.5% of a column volume) without affecting chromatographic separations or carrying compounds with it.
Tags: Posters
New Flash System Capabilities Improve Purification Times and Performance
New, higher default flow rates enabled by Isolera ACI systems decreases purification times compared to other systems’ and cartridges’ default flow rate settings without sacrificing separation efficiency. Incorporation of gradient optimization by converting a linear gradient to a step gradient further reduces purification time and solvent use while maintaining separation integrity for a target molecule.
Tags: Posters
Mass-directed Flash Purification of Peptides
In this poster we demonstrate real-time detection and identification of peptides during reversed-phase flash chromatography with the new Biotage® Isolera™ Dalton mass-directed flash purification system.
Presented at ACS Spring 2014.
Tags: Posters
Time-Efficient Chemistry: Rapid Routes from Synthesis to Isolated Compound (II)
In order to obtain a fast synthesis of an N-unprotected tripeptide, microwave (mw) assisted deprotection, coupling and partial deprotection were applied starting with a purified protected dipeptide [Fig. 1]. The three consecutive mw assisted steps were performed without any intermediate purifications and were accomplished within some 30 minutes. The resulting crude mixture containing the Boc deprotected tripeptide ester was shown to be quite complex [Fig. 2]. The crude product was subjected to automated reversed phase flash chromatography, followed by automated fraction pooling and “thin film evaporation”. The total time for the entire procedure was 1 h 20 min. The purity of the product was > 99%.
Tags: Posters
» PRODUCT NOTES
ACI™ Accelerated Chromatographic Isolation
With running costs and time a concern in most labs, ACI™ now promises to take users to the end point even faster and even more efficiently.
Tags: Product Notes
Isolera™ Leak Detection System
The Isolera leak detection system is designed with safety in
mind. Unlike vapor sensing devices with unreliable solvent
vapor sensitivity, the Isolera leak detector’s sensitive,
solvent-resistant refractive index (RI) monitor rapidly
signals the Isolera flash system to stop pumping if any
liquid, volatile or non-volatile, is detected thus protecting
the chemist, system, and lab from potential hazards.
Tags: Manuals & User Guides,
Product Notes
» RELEASE NOTES
» SOFTWARE
Adduct and Fragment Calculator
Use this simple tool to look up possible adducts and fragments which may occur with Isolera™ Dalton or Biotage® Dalton 2000 mass detectors.
Enter the target compound weight and use the mass lookup cell to highlight resulting m/z values.
Tags: Software
Search all documents »
INSTRUCTION VIDEOS
Biotage® Isolera™ Spektra
Isolera™ Dalton
Isolera™ Tutorial
WEBINARS
Isolera™ Spektra - A Revolution in Intelligent Flash Chromatography
Reversed Phase Purification: Bridging the Gap From Flash to Prep HPLC
News
13 July 2017New Peptide Synthesis Workflow Brochure
Biotage is pleased to announce the release of the new Peptide Synthesis Workflow – Synthesis, Purification, and Evaporation Solutions Brochure The typical peptide synthesis workflow broadly ...
24 May 2017Biotage Releases Isolera™ Dalton 2000
Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, is pleased to announce the launch of Isolera™ Dalton 2000, a new ma...
12 September 2014Isolera™ Used in Recent Natural Products Study
The capabilities of Biotage flash chromatography systems for providing pure compounds from natural products is confirmed in an extensive Korean study of active compounds in the medicinal herb&...
09 September 2014Webinar on Flash Purification Evolved: ACI™ Accelerated Chromatographic Isolation
ACI™ - Accelerated Chromatographic Isolation radically improves the efficiency of the laboratory, embracing the latest developments in purification technology to guide chemists towards improv...
07 August 2014Find Out Why The Era of Regular Flash Has Come To An End
Flash Purification Has Come of Age Flash purification is a chromatographic technique developed with one goal in mind – to deliver the maximum amount of purified product in the minimum ...
27 June 2014Biotage Launches ACI™ - Accelerated Chromatographic Isolation
Uppsala, Sweden, 27th June, 2014 – Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, announces the launch of ACI&trad...
14 March 2014The Lines Between Purification and Analysis are Blurring…
By Dr. Greg Saunders, EU Marketing Manager for Organic and Peptide Chemistry at Biotage Liquid chromatography (LC) is used for two different purposes – analytical work and purification &ndas...
11 February 2013TLC-to-Step Gradient capability significantly reduces both purification time and solvent use
Uppsala, Sweden, 1st February, 2013 Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, has announced the launch of an updat...
04 September 2012Biotage moving into Mass Directed Flash
Uppsala, Sweden, 4th September, 2012 Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, announces that Biotage is in late s...
15 June 2012Advanced Chromatography Explained
Dr. Sunil Rana, product manager at Biotage, explains the advanced features for flash chromatography in the new Isolera™ Spektra release. Watch the free webinar recording here >
26 April 2012New Isolera™ Spektra instruction video
23 April 2012 Product manager Bob Bickler introduces the new time-saving Isolera™ Spektra features for Isolera One, Four and LS Flash chromatography instruments.
18 October 2011Purifying the First Hundred Grams
Preparative FLASH chromatography expedites the production of clinical trial compounds. Tobi Williams is an application chemist specializing in pilot and production purification at Biotag...
16 August 2010New Isolera™ UV-VIS Flash Purification System delivers widest wavelength range available to chemists
Charlotte, North Carolina, USA, August 16, 2010 – Biotage (STO: BIOT), a leading developer of tools and technology for medicinal and analytical chemistry, announces the introduction of the Is...
21 April 2010Biotage Awarded Patent for Flash Purification
Uppsala, Sweden, April 20, 2010 – Biotage (STO: BIOT), a global leader in automated flash purification, tools and technology for medicinal and analytical chemistry, announced it has recently ...
23 February 2010Biotage Raises the Bar in Flash Purification with Technology Dramatically Reducing Solvent Use and Costs up to 60%
Uppsala, Sweden, February 23, 2010 – Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, announced the launch of enhanced gradient optimization (GO) te...
27 October 2008Biotage Introduces a New Synthesis and Purification Catalog
October 28, 2008 Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, announces the release of its new 2008–09 Synthesis and Purification Catalog. The ...
INSTRUMENTS
| Part No. | Description | Product type | Pack Size | Price | |
| DALT-2000-APCI-100V | Biotage Dalton 2000 Mass Detector APCI 100 VAC incl roughing pump | Instruments | 1 | Log in for price | |
| DALT-2000-APCI-115V | Biotage Dalton 2000 Mass Detector APCI 115 VAC incl roughing pump | Instruments | 1 | Log in for price | |
| DALT-2000-APCI-230V | Biotage Dalton 2000 Mass Detector APCI 230 VAC incl roughing pump | Instruments | 1 | Log in for price | |
ACCESSORIES
| Part No. | Description | Product type | Pack Size | Price | |
| DALT-2000-NLINK | Biotage Dalton 2000 Nanolink | Accessories | 1 | Log in for price | |
| IS-ESI-S01 | Dalton 2000 ESI Ion source | Accessories | 0 | Log in for price | |
The Isolera™ Dalton 2000 system consists of Biotage® Dalton 2000 Mass Detector, Isolera™ Dalton Nanolink and Isolera™ Spektra and embraces ACI™ for efficient and automated purification. Isolera Dalton 2000 directs flash fractionation by molecular mass, and in real time.
Expanded functionality
Biotage® Dalton 2000 is a compact single quadrupole mass detector with simultaneous positive and negative polarity switching. Biotage® Dalton 2000 uses an atmospheric pressure chemical ionization source to ionize substances, which can then be identified by their mass-to-charge ratios (m/z). Biotage® Dalton 2000 allows you to analyze samples and automatically use the m/z ratio(s) of the identified substance(s) for flash purification.
A novel function, 'Collect on TIC', monitors the entire specified range of m/z values. This is beneficial for samples that easily form fragments and/or adducts, where peaks are therefore found on several m/z values, or if the sample contains many different components.
Tailored Technology
Isolera™ Dalton 2000 automates and takes the hard work out of the compound identification and purification, perfect for chemists whose priorities lie more towards the chemistry than small details of running the analysis. Minimal user intervention required makes it available to chemists unfamiliar with mass detection or those whose priorities lie in compound synthesis rather than analysis.
Intelligent Integration
Seamless interaction between a flash system and a mass detector requires advanced technology and fine tuning. This is all taken care of by the Isolera™ Dalton Nanolink interface, an intelligent sampling device that at any given moment provides Biotage® Dalton 2000 Mass Detector with the exact amount of sample required, and dynamically adjusts to the solvent effluent flow rate employed in the Flash system.
Isolera™ Spektra software with its graphic user interface provides a straightforward controlling environment, giving easy access to the process.
Advantages
- Compound identification by mass during purification and by direct injection analysis
- Fully automated integration of Flash purification and mass detection via the Isolera™ Dalton Nanolink
- Automatically accommodates different flash flow rates
- Simple wizard-based approach to method development
