The method described in this application note demonstrates selective extraction of the thyroid hormones T3, rT3 and T4 from serum. 200 µL of serum was extracted using the EVOLUTE® EXPRESS AX 30 mg fixed well plate format. High reproducible recoveries and low phospholipid content were observed, demonstrating limits of quantitation < 50 pg/mL.
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Application Video: Extraction Steroids from Serum with ISOLUTE® SLE+
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