Standard HPLC-mediated purification is often the most time consuming aspect of the peptide synthesis workflow. Medium pressure chromatography is gaining traction as a complement to traditional HPLC purification methods for a variety of reasons. While the larger particles greatly increase the overall loading capacity per injection, reducing the overall purification time, this comes with a cost of resolution during chromatography.
In this event we’ll highlight several strategies that can be implemented to improve the final peptide purity, despite the low pressure and large particle size, potentially eliminating the need for HPLC purification altogether.
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