This application note describes the extraction of 96 licit and illicit drugs of abuse from urine prior to UPLC-MS/MS analysis using EVOLUTE® HYDRO CX 96-well plates. EVOLUTE® HYDRO CX plates offer an efficient way to perform hydrolysis in the well of the extraction plate. This method provides high analyte recovery, reduced extraction time due to the elimination of a sample transfer step as well as the elimination of the column conditioning and equilibration steps, and a reduced risk for sample carryover or cross-contamination due to the elimination of the sample transfer step.

6-acetyl codeine 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam 96-well plate Alprazolam Amitriptyline Amphetamine Amphetamines Analgesics Application Notes Aripiprazole Atomoxetine Barbiturates Benzodiazepines Benzoylecgonine Buprenorphine Bupropion Buspirone Butalbital Carbamate hypnotic Carbamate muscle relaxant Carbamates Carbazepine Carisoprodol Chlordiazepoxide Chlorpheniramine Chlorpromazine Clinical Clomipramine Clonazepam Clonidine Clozapine Cocaethylene Cocaine Cocaines Codeine Cyclobenzaprine Desalkylflurazepam Designer stimulants Dextromethorphan Diazepam Dihydrocodeine (DHC) Drugs of Abuse Duloxetine EDDP English Ethyl Sulfate (EtS) Extrahera Fentanyl Fluoxetine Forensic Gabapentin Haloperidol Hydrocodone Hydrolysed urine Hydromorphone Hydroxyalprazolam Hydroxybupropion Hydroxymidazolam Hydroxytriazolam Imipramine Ketamine LC-MS/MS Lamotrigine Levetiracetam Lidocaine Lorazepam MDA MDEA MDMA Meperidine Meprobamate Methadone Methamphetamine Methaqualone Methcathinone Midazolam Morphine N-des-tapentadol N-desmethylclomipramine N-desmethyltapentadol Naloxone Norbuprenorphine Nordiazepam Norfentanyl Norhydrocodone Norketamine Normeperidine Noroxymorphone Norpropoxyphene Nortryptiline Opiates Opioid Opioid agonist Oxazepam Oxcarbazepine Oxycodone Oxymorphone Pain Management Pentobarbital Pharmaceuticals Phencyclidine (PCP) Phenobarbital Pregabalin Quetiapine Ritalinic acid Secobarbital Stimulants THC-COOH Tapentadol Temazepam Topiramate Tramadol Triazolam Trimipramine Urine Venlafaxine Zolpidem Zolpidem-phenyl-4-COOH mCPP z-drugs

This is the importable .exp method for the application note AN842

25-OH Vitamin D2 25-OH Vitamin D3 25-hydroxy-vitamin D Extrahera

This is the importable .exp method for the application note AN847

Extrahera MMA (Methylmalonic acid) Methylmalonic acid (MMA)

This is the importable .exp method for the application note AN847

Extrahera MNZOH Methylmalonic acid (MMA)

This is the importable .exp method for the application note AN849

Extrahera MMA (Methylmalonic acid) Methylmalonic acid (MMA)

This is the importable .exp method for the application note AN850

Extrahera MMA (Methylmalonic acid) Methylmalonic acid (MMA)

This is the importable .exp method for the application note AN851

Extrahera MMA (Methylmalonic acid) Methylmalonic acid (MMA)

This is the importable .exp method for the application note AN857

1, 25 Dihydroxyvitamin D 1a, 25-dihydroxy-vitamin D 1α, 25-dihydroxy-vitamin D Extrahera

This is the importable .exp method for the application note AN861

Extrahera MMA (Methylmalonic acid) Methylmalonic acid (MMA)

This application note describes the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. The method was automated using Biotage® ExtraheraTM, configured for use with EVOLUTE EXPRESS ABN columns. The method delivers clean extracts and analyte recovery of 90% with RSD lower than 10% for carboxy-THC and its deuterated internal standard. Using the Biotage® ExtraheraTM, 24 samples were extracted in approximately 38 minutes. The limit of quantitation of 2 ng/mL is below the SAMHSA/ EWDTS confirmation cut off of 15 ng/mL for workplace testing applications.

11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) Application Notes Column English Extrahera Forensic GC-MS Hydrolysed urine THC metabolites THC-COOH

This application note describes the operating conditions for the automated fractionation of Extractable Petroleum hydrocarbons (EPH) into aliphatic and aromatic (PAH) fractions using the ISOLUTE EPH SPE column in conjunction with the Extrahera automation system.

Acenaphthene Acenapthalene Aliphatic hydrocarbons Anthracene Application Notes Aromatic hydrocarbons Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(ghi)perylene Benzo(k)Fluoranthene Chrysene Column Dibenzo(ah)anthracene English Environmental Extrahera Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene Napthalene Phenanthrene Polyaromatic Hydrocarbons (PAH) Pyrene Soil

This document details an example of the pipetting precision and accuracy data for four specified volumes across 24 (4 x 6) positions.

Clinical Column English Extrahera Forensic Gravimetric Pharma Product Notes

This document details an example of the pipetting precision and accuracy data for four specified volumes across a full 96 well plate.

96-well plate Clinical English Extrahera Forensic Gravimetric Pharma Product Notes

This poster compares the use of supported liquid extraction (ISOLUTE® SLE+) and a novel protein and phospholipid depletion plate (ISOLUTE® PLD+), for the extraction of 25-hydroxy vitamin D. The manual extraction protocols were transferred to an SPE automation platform (Biotage® Extrahera)and method performance versus manual processing compared. MSACL EU 2015

25-OH Vitamin D2 25-OH Vitamin D3 25-hydroxy-vitamin D 96-well plate Clinical English Extrahera LC-MS/MS Posters Serum Vitamin D Vitamins

This product sheet compares automated sample preparation using the Biotage®Extrahera™ to an equivalent manual method utilizing a vacuum manifold. A selection of beta blocker drugs were extracted from pooled stripped plasma using a supported liquid extraction procedure.

96-well plate Alprenolol Beta blockers Clinical English Extrahera Forensic LC-MS/MS Metoprolol Pharma Pindolol Plasma Product Notes Propranolol β-blockers

Automated sample preparation using the Biotage®Extrahera™ was compared to an equivalent manual method utilizing a vacuum manifold. Analytes were extracted from pooled stripped plasma using a supported liquid extraction procedure.

25-OH Vitamin D2 25-OH Vitamin D3 25-hydroxy-vitamin D 96-well plate Clinical English Extrahera LC-MS/MS Plasma Product Notes Vitamin D

This document compares automated sample preparation using the Biotage® Extrahera™ to an equivalent manual method utilizing a vacuum manifold. A selection of non-steroidal anti-inflammatory drugs (NSAIDS) were extracted from pooled stripped plasma using a supported liquid extraction procedure.

96-well plate Clinical English Extrahera Forensic Indomethacin Ketoprofen LC-MS/MS NSAIDs Pharma Plasma Product Notes Sulindac

This poster discusses the potential for a single extraction protocol for various drugs of abuse classes in whole blood prior to UPLC-MS/MS analysis. SOFT 2017, Boca Raton also presented at SFTA, Marseille, France, 2018

Amphetamines Benzodiazepines Cannabinoid Cathinones Clinical Cocaines Column Drugs of Abuse English Extrahera Forensic LC-MS/MS Opiates Posters THC metabolites Whole blood z-drugs

This poster demonstrates that a large urine panel, comprised of 43 DOAs, from multiple drug classes, can be simultaneously screened by mixed-mode cation exchange SPE (using EVOLUTE EXPRESS CX 96 well plates) despite their disparate intermolecular traits, by thoughtfully selecting appropriate organic wash and elution conditions that simultaneously enable sample isolation and detection along with minimizing sample matrix effects. The extraction method is automated using the Biotage® Extrahera™ Automated sample Preparation Platform. MSACL 2017, Palm Springs SOFT 2017, Boca Raton

96-well plate Benzodiazepines Carbamates Carisoprodol Clinical Drugs of Abuse English Extrahera Forensic Gabapentin Hydrolysed urine LC-MS/MS Meprobamate Opiates Pain Management Posters Pregabalin Stimulants Urine

This poster demonstrates the use of a novel protein and phospholipid depletion plate, for the extraction of 25-hydroxy vitamin D from serum. The extraction protocol was ultimately transferred to an SPE automation platform and method performance versus manual processing was compared. ASMS 2015

25-OH Vitamin D2 25-OH Vitamin D3 25-hydroxy-vitamin D 96-well plate Biomarker Clinical English Extrahera LC-MS/MS Posters Serum Vitamin D Vitamins